Inactivation and Regeneration of Immobilized Catalase

Catalase was immobilized on collagen membrane. The inactivation of immobilized catalase by 0.01M and 0.1M H 2 O 2 was reported. After the initial stage of inactivation, a stable catalatic activity as measured in a continuous flow of 0.01M H 2 O 2 through a modular reactor was observed for longer than 20 days (2.6 min residence time). The regeneration of catalatic activity from the O.1M H 2 O 2 inactivated catalase occurred after incubating the inactivated modular reactor with 0.01M phosphate buffer, pH 6.8. The amount of activity regenerated is directly proportional to the time of incubation.