Industrial wastes as substrates for synthesis of surfactants with antiadhesive activity by Rhodococcus erythropolis IMV Ac-5017

Introduction.Microbial surfactants can affect the degree of microorganism's adhesion to different surfaces and lead to biodegradation of the already formed biofilms.They are also non-toxic to the environment.These properties allow to use the microbial surfactants in medicine and food industry. Material and methods.Surfactants were obtained by cultivation of the Rhodococcus erythropolis IMV Ac-5017 on waste from biodiesel production, used sunflower oil after frying meat, potatoes, onions, and cheese.The surfactants were extracted from the supernatant of cultural liquid by a Folch mixture.The anti-adhesive activity and the degree of destruction of biofilms were determined by spectrophotometric method.Results and discussion.It was found that surfactants obtained by cultivation of R. erythropolis IMV Ac-5017 on waste mixed sunflower oil reduce the adhesion of yeast Candida albicans D-6, Candida utilis BVS-65, Candida tropicalis PE-2 and bacterial strains Escherichia coli IEM-1, Bacillus subtilis BT-2, Pseudomonas sp.MI-2 to abiotic surfaces (tiles, steel and glass) by 51-73% and 57-86%, respectively.The effectiveness of the anti-adhesive activity of surfactants, obtained on waste from biodiesel production as a substrate against the studied yeast and bacterial cultures was lower (44-77%).The ability of surfactants to destroy of bacterial biofilms by 44-73% was achieved at low concentrations of surfactants (8-15 μg/ml) synthesized on different waste oil.Surfactants were equally effective in destroying biofilms (72-80%) of Candida genus and their activities were not depended on the nature of carbon source in the culture medium of R. erythropolis IMV Ac-5017. Conclusion.The obtained results show the possibility of using surfactants of R. erythropolis IMV Ac-5017, gained on industrial waste, as effective anti-adhesive and antibiofilm agents.