Background: One of the new emerging techniques to preserve reproductive potential of cancer patients is cryopreservation of ovarian fragments prior to medical treatment and their re-transplantation after healing. In order to investigate and compare apoptosis in ovarian tissue, the current study has done while using different vitrification solutions. Methods: The ovaries of Balb/c mice were removed from the body. R ovaries autotransplanted and L ovaries vitrified and thawed in different solutions. Apoptosis-related genes (Bax, Bcl-2, Bid, P53) assessed by RT-PCR. Results: The results have shown that Bax and P53 expression showed significant changes in DCV3, but in DCV2 only p53 expression decreased considerably in vitrified-thawed group. Conclusion: We concluded that cryoprotectant solution is important for preserving tissue in vitrification process and in our study DCV2 showed better results than DCV3.
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Izadpanah et al. (2023) studied this question.
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