Synthesis of silver nanoparticles using 3,5-di-t-butyl-4-hydroxyanisole from Cynodon dactylon against Aedes aegypti and Culex quinquefasciatus

Emergence of resistance among mosquitoes is recent problem. In the present study, was mosquito larvicidal activity of synthesized silver nanoparticles (Ag NPs) 3,5-di- t -butyl-4-hydroxyanisole isolated from Cynodon dactylon against the first to fourth instar larvae dengue vector, Aedes aegypti and filariasis vector, Culex quinquefasciatus (Diptera: Culicidae) was carried out. The synthesized Ag NPs were characterized by UV, XRD and HR-TEM. The HR-TEM analyses were spherical shaped. The highest mortality of synthesized Ag NPs tested against first to fourth instars of A. aegypti (LC 50 = 2.50, 2.78, 3.02, 3.05 μg/mL; LC 90 = 8.28, 7.47, 8.13, 8.74 μg/mL) and C. quinquefasciatus (LC 50 = 2.75, 3.00, 3.21, 3.48 μg/mL; LC 90 = 6.45, 7.72, 11.06, 12.83 μg/mL), at 10 μg/mL, respectively. Synthesized Ag nanoparticles were effective as potential anti larvicidal agent against Aedes aegypti and Culex quinquefasciatus mosquitoes.