The use of an ion‐exchange resin for tissue hydrolysis in the determination of hexosamine

Hydrolysis of tissue samples with Dowex‐50 resin suspended in 0.05N‐hydrochloric acid, with subsequent elution of the hexosamine from the resin, afforded water‐clear solutions containing the hexosamine. The hexosamine in this eluate could be determined directly by a modification of the method of Elson & Morgan, thus reducing or avoiding the necessity of correcting for interference by interactions between sugars and amino‐acids and by humin. The technique described gave results about 10% lower than those obtained by the modified method previously described by the authors.