Protein-confined bimetallic structural ensembles with mimetic peroxidase activity are constructed. The oxidation reaction of 3,3,5,5-tetramethylbenzidine (TMB) with H2O2 is chosen to detect catalytic activity using UV–vis spectroscopy. The artificial enzyme is coupled with oxidase to build a dual-enzyme sensor system for the detection of biomolecules.
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Zhang et al. (2012) studied this question.
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