Long‐term results with organ cultured, cryopreserved human corneal grafts. Re‐examination of 17 patients

Seventeen organ cultured cryopreserved corneas were grafted in 1978-79. When published in 1982, 12 of the grafts (71%) were clear with an average thickness of 0.51 mm and an endothelial density of 1028 cells/mm2. A re-examination of these patients after 13 years is presented. Seven grafts (58%) were clear. Average CCT was 0.51 mm and endothelial cell density was 988 cells/mm2. Visual acuity was 0.4 or better. The morphology resembled that seen after 1 year with large, multinucleated cells. It is concluded that cryopreserved grafts show a fair long-term survival. Cryopreservation may still present a possibility in the establishment of a corneal bank with the perspective of supplying histocompatible donor material.