Key result
Pentoxifylline treatment significantly improved diabetes-induced myocardial damage and restored nNOS and iNOS expressions in the heart tissue of diabetic rats.
Why the study?
Does pentoxifylline improve nitric oxide synthase expression and histological damage in the heart tissue of streptozotocin-induced diabetic rats?
Does pentoxifylline improve nitric oxide synthase expression and histological damage in the heart tissue of streptozotocin-induced diabetic rats?
Absolute Event Rate: 81.32% vs 75.54%
p-value: p=0.049
Pentoxifylline ameliorates diabetic myocardial damage in rats by reducing nitrosative stress and improving nNOS and iNOS expression.
May inform diabetic cardiomyopathy therapies; leaves open translation from rat models to humans.
OBJECTIVE: Diabetes mellitus causes a decrease in cardiac output, arterial blood pressure, and heart rate. In this study, we aimed to investigate, at the molecular level, the effect of nitric oxide synthase (NOS) on heart pathology in type 1 diabetes and look at the therapeutic effect of pentoxifylline on this pathology. METHODS: In this experimental study, 50 Wistar albino male rats were used. The rats were divided into 5 groups: group C, control; group D, only diabetes; group D+PI and D+PII, diabetes + pentoxifylline; group P, only pentoxifylline. Group D+PI rats received 50 mg/kg/day pentoxifylline over two months. However, group D+PII rats received saline in the first month and 50 mg/kg/day of pentoxifylline over the following month. At the end of two months, NOS expressions in heart tissue were assessed through immunohistochemistry analysis. The data were compared by one-way ANOVA. RESULTS: At the end of the experiments, there was increased cytoplasmic vacuolization, myofibrillar loss, cytoplasmic eosinophilia, and degeneration of cardiomyocytes; nNOS and iNOS expressions in group D decreased compared with that in group C. In group D+PI and group D+PII, nNOS and iNOS expressions improved compared with group D. CONCLUSION: As a result, we found that diabetes, a known chronic disease, causes serious damage in heart tissue. NOS plays a role in this damage, and pentoxifylline aided in improving nNOS and iNOS expression in this damage.
No takes yet. Share an insight, caveat, or question.
Karabulut et al. (2015) studied Type 1 Diabetes Mellitus and Diabetic Cardiomyopathy (n=50). Pentoxifylline vs. Saline was evaluated on nNOS expression in heart tissue (histoscore) (p=0.049). Pentoxifylline treatment significantly improved diabetes-induced myocardial damage and restored nNOS and iNOS expressions in the heart tissue of diabetic rats.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: