By means of a particular definition of the “compensated extinction”, it is possible, with the help of only one calibration curve, to determine the concentration of one substance when there is some overlapping between the characteristic band of this substance and the spectra of the other constituents of the mixture. This method was applied to the determination of γ.hexachlorocyclohexane in the presence of the α, β an δ isomers.
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Gérard Pirlot (1950) studied this question.
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