Surface topography of a biomaterial plays an essential role in the regulation of cell adhesion, proliferation, and differentiation. To investigate the influence of hierarchically micro/nano‐structured substrates on the differentiation of mesenchymal stem cells (MSCs), a series of micro‐, nano‐, and micro/nano‐structured titanium substrates were fabricated via dual acid etching and/or anodic oxidation. The morphologies of titanium substrates were characterized by field emission scanning electron microscopy (FE‐SEM) and contact angle measurement, respectively. The differentiation of MSCs adhered to different substrates was investigated in vitro. Compared to native titanium, micro/nano‐structured titanium substrates improved the alkaline phosphatase (ALP) production and mineralization of MSCs. More importantly, micro/nano‐structured titanium substrates demonstrated great potential to induce the differentiation of MSCs, which was revealed by the expressions of osteocalcin (OCN) and osteopontin (OPN). The study provides a potential alternative to generate hierarchically micro/nano‐structured titanium‐based implant for enhanced osseointegration.
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Chen et al. (2011) studied this question.
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