We have examined the separation of test mixtures by linear and step temperature gradients in open tubular capillary liquid chromatography, in reverse and normal-phase modes. Separations using temperature gradients with the upper temperature limit of 433 K were compared to isothermal runs in terms of chromatographic efficiency, analysis time and selectivity. When a temperature gradient (363 K to 433 K in 12 min) was utilized in reverse phase separation of chlorobenzenes, a 40% reduction in analysis time, compared to an isothermal separation at 363 K, was accomplished.
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Ryan et al. (1996) studied this question.
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