A pulsed-field gel electrophoretic method based on contour-clamped homogeneous electric field (CHEF) was developed for the analysis of natural isolates of Rhizobium leguminosarum biovar viciae. The procedure involves the use of ‘rare-cutting’ endonucleases. The separation of genomic DNA fragments with split runs (τ= 5 s for 15 h followed by τ= 12 s for 9 h) allows a clear definition of profiles with bands ranging from 20–300-kb pairs. Two and a half days are sufficient to reproducibly accomplish the procedure from cell lysis to gel picture. The method can be used for different fast-growing rhizobia.
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Corich et al. (1991) studied this question.
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