SYNOPSIS. An analysis of the pigments from the protozoan Blepharismn undtulans is reported. Methods of isolation and separation by paper chromatography are described. along with subsequent spectrophotometric and chemical analysis. Since the two major pigments were available only in amounts insufficient for thorough chemical analysis, these pigments were compared with the known pigment hypericin. Although similarities in the visible spectra indicate that the B. unduluns pigments possess the same mesodianthrone structure as hypericin, infrared spectra and chemical reactions disclose differences in the position, and possibly in the number, of the functional groups. A tentative structure is proposed.
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M. R. Sevenants (1965) studied this question.
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