The Rh antigen activity of human erythrocyte membranes was lost following extraction with 100% 1-butanol, but could be regenerated by the addition of the membrane extract or of a chloroform-methanol extract of human plasma. Studies of the extract by silicic acid and diethylaminoethyl cellulose chromatography, thin layer chromatography, and digestion with snake venom indicate that the active component is phosphatidylcholine. Preliminary data suggest that the lecithin must contain unsaturated fatty acids.
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F A Green (1968) studied this question.
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