Enzyme‐assisted aqueous extraction of rice bran oil

In the present study, rice brain oil was extracted by enzyme‐assisted aqueous extraction under optimized aqueous extraction conditions using mixtures of ProtizymeTM (protease; Jaysons Agritech Pvt. Ltd., Mysore, India), PalkodexTM (α‐amylase; Maps India Ltd., Ahmedabad, India), and cellulase (crude cellulase; Central Drug House, Delhi, India). The optimal conditions used were: mixtures of amylase (80 U), protease (368 U), and cellulase (380 U), with 10 g of rice brain in 40 mL distilled water, pH 7.0, temperature 65°C, extraction time 18 h with constant shaking at 80 rpm. Centrifugation of the mixture at 10,000×g for 20 min yielded a 77% recovery of the oil.