Polyphasic taxonomic study identifies novel actinobacterium Planosporangium spinosum from tropical peat swamp soil, expanding known bacterial diversity within the genus Planosporangium.
A novel actinobacterium, designated strain 12N6 T , was isolated from tropical peat swamp forest soil in Rayong Province, Thailand. The taxonomic position was determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 12N6 T was classified within the genus Planosporangium and showed the highest percentage similarity to Planosporangium flavigriseum YIM 46034 T (98.7%), followed by Planosporangium thailandense HSS8-18 T (98.0%) and Planosporangium mesophilum YIM 48875 T (97.9%). Strain 12N6 T produced a single globose spore with a spiny surface on short sporophores of the substrate mycelia. The approximate genome size and DNA G+C content of strain 12N6 T were 7.47 Mbp and 71.6 mol%, respectively. The highest average nucleotide identity and digital DNA–DNA hybridization values of genome sequences of 12N6 T compared with closest species type strains (83.0% and 27.8%, respectively) are well below the thresholds for species delineation. The whole-cell hydrolysates of strain 12N6 T contained meso -diaminopimelic acid, glucose, mannose, arabinose, xylose and ribose. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major cellular fatty acids were anteiso-C 17 : 0 , iso-C 16 : 0 and iso-C 15 : 0 . The analysis of phylogenetic, genomic, phenotypic and chemotaxonomic characteristics revealed that strain 12N6 T is considered to represent a novel species of the genus Planosporangium , for which the name Planosporangium spinosum sp. nov. is proposed. The type strain is 12N6 T (=TBRC 19149 T =NBRC 117121 T ).
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Tongtoht et al. (2025) studied this question.
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