Key result
Antisera containing 4% polyethylene glycol 6000 permitted a fourfold dilution for use in the capillary precipitin test while exceeding potency requirements.
Why the study?
Does the addition of 4% polyethylene glycol 6000 to streptococcal grouping antisera improve the precipitin reaction?
Does the addition of 4% polyethylene glycol 6000 to streptococcal grouping antisera improve the precipitin reaction?
The addition of 4% polyethylene glycol 6000 to streptococcal grouping antisera enhances the precipitin reaction, allowing for a fourfold dilution while maintaining required potency.
Answers are grounded in this paper and cite their sources.
May improve streptococcal grouping assay efficiency; leaves open clinical diagnostic adoption pending validation.
Antisera to streptococcal groups A through G containing 4% polyethylene glycol 6000 were prepared and evaluated. Seventy strains of homologous and heterologous beta- and non-beta-hemolytic streptococci were included in the evaluation. Homologous reactions were determined against extracts prepared by four extraction methods: hot hydrochloric acid (Lancefield) extraction, autoclave extraction, hot formamide extraction, and nitrous acid extraction. Enhancement of the precipitin reaction in the presence of polyethylene glycol 6000 permitted a fourfold dilution of all antisera for use in the capillary precipitin test. At this dilution, the potency of the antisera exceeded requirements established for these reagents.
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George et al. (1981) studied Streptococcal grouping (n=70). Antisera containing 4% polyethylene glycol 6000 was evaluated on Enhancement of the precipitin reaction and potency of antisera. Antisera containing 4% polyethylene glycol 6000 permitted a fourfold dilution for use in the capillary precipitin test while exceeding potency requirements.
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