Structural Analysis of the Glycine-Rich, Arginine-Rich Histone from Calf Thymus: The Tryptic Peptides

The glycine-rich, arginine-rich histone from calf thymus contains 102 residues of amino acids, distributed as follows: Asp5, Thr7, Ser2, Glu6, Pro1, Gly17, Ala7, Val9, Met1, Ile6, Leu8, Tyr4, Phe2, Lys10, Lys(Me)1, His2, Arg14. From a tryptic hydrolysate of Gly- and Arg-rich histone, 18 peptides were isolated by fractionation on a column of Chromobeads P followed by purification by paper electrophoresis and chromatography. The amino acid compositions of these peptides are reported. The sequence of 12 of these tryptic peptides has been established. The amino-terminal tryptic peptide is Ac-Ser-Gly-Arg. The carboxylterminal tryptic peptide is Thr-Leu-Tyr-Gly-Phe-Gly-GlyCOOH.