The H and I antigens were studied in the plasma and saliva of 52 group O subjects, by hemagglutination inhibition at continuous flow. H plasma substance was detected with one anti-H antibody from a Bh donor. Secretors have more H plasma substance than do non-secretors, but there was no relationship between the amounts of I and H substances in the plasma. On the contrary, the amounts of I substance in the saliva detected by anti-I (Sti) is a function of secretor status. Nonsecretors had a much greater amount of I in saliva than did secretors. These results support the hypothesis that I antigen (detected by anti-I (Sti)) is a part of the substrate of H antigen.
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Rouger et al. (1980) studied this question.
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