Key result
RT-PCR detected pestivirus contamination in 15 of 20 (75%) mammalian cell lines, including bovine diarrhea virus and border disease virus.
A high rate (75%) of pestivirus contamination, primarily BVDV, was found across various mammalian cell lines, highlighting the need for rigorous screening in cell culture work.
Pestivirus contamination threatens cell line experiment validity; leaves open standardized screening needs in cardiovascular research models.
We examined 20 cell lines of various animal origins for the presence of pestivirus contamination by the reverse transcription-polymerase chain reaction (RT-PCR), and found 15 (75%) cell lines were positive. The RT-PCR products of the 5' untranslated region (UTR) of pestivirus genome were sequenced and subjected to genotyping. Stem-loop structures at three variable regions in the 5' UTR render genotyping of the contaminated pestiviruses. Bovine cell lines tested were all contaminated with genotypes I, II, or III of bovine diarrhea virus (BVDV). Cell lines of canine, feline, and primate origin were contaminated with genotype II of BVDV. Cell line Ch1Es of caprine origin was contaminated with border disease virus (BDV).
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Harasawa et al. (1995) studied Pestivirus contamination (n=20). RT-PCR and genotyping was evaluated on Presence of pestivirus contamination. RT-PCR detected pestivirus contamination in 15 of 20 (75%) mammalian cell lines, including bovine diarrhea virus and border disease virus.
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