Key result
Polypeptides and polyphenols in Chinese rice wine reduced homocysteine-induced proliferation and migration of vascular smooth muscle cells and decreased the expression and activity of MMP-2/9.
Why the study?
Do polyphenols and polypeptides from Chinese rice wine inhibit homocysteine-induced proliferation and migration in vascular smooth muscle cells?
Do polyphenols and polypeptides from Chinese rice wine inhibit homocysteine-induced proliferation and migration in vascular smooth muscle cells?
Polypeptides and polyphenols in Chinese rice wine inhibit homocysteine-induced proliferation and migration of vascular smooth muscle cells, suggesting a potential mechanism for its cardiovascular protective effects.
May support mechanistic studies on dietary polyphenols; hypothesis-generating and should not change practice.
The beneficial effect of Chinese rice wine on atherosclerosis has been proved, but the exact components that have the cardiovascular protective effect are still unknown. This study aimed to explore the exact ingredients in Chinese rice wine that could inhibit homocysteine (Hcy)-induced vascular smooth muscle cell (VSMC) proliferation and migration. VSMCs were divided into 7 groups: control, Hcy (1 mmol/L), Hcy + oligosaccharide, Hcy + polypeptides, Hcy + polyphenols, Hcy + alcohol, and Hcy + Chinese rice wine. methyl thiazolyl tetrazolium (MTT) assay, Transwell chambers, and wound-healing assay were used to test the proliferation and migratory ability of the VSMCs. Western blot and gelatin zymography were used to investigate the expressions and activities of metal matrix proteinase 2/9 (MMP-2/9) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in VSMCs. Polypeptides and polyphenols in the Chinese rice wine reduced the proliferation and migration ability of the VSMCs. Furthermore, they also decreased the expression and activity of MMP-2/9 but had no obvious impact on the expression of TIMP-2 in each group. This study further confirms that polypeptides and polyphenols in the Chinese rice wine could inhibit Hcy-induced proliferation and migration of VSMCs and maintain the balance between matrix metalloproteinases (MMPs) and TIMPs.
No takes yet. Share an insight, caveat, or question.
Meng et al. (2016) studied Homocysteine-induced vascular smooth muscle cell proliferation and migration. Polypeptides and polyphenols in Chinese rice wine vs. Control, Hcy alone, Hcy + oligosaccharide, Hcy + alcohol was evaluated on Proliferation and migration of VSMCs, expression and activity of MMP-2/9 and TIMP-2. Polypeptides and polyphenols in Chinese rice wine reduced homocysteine-induced proliferation and migration of vascular smooth muscle cells and decreased the expression and activity of MMP-2/9.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: