Deficiency in Ca and P in mineral medium reduced the growth and nitrogen content of Azolla pinnata but increased the content of soluble sugars in the plant; the deficiency in K and Mg was less effective in retarding the growth. The pH of the medium from 5 to 8 encouraged the growth and nitrogen fixation, whereas acidic pH of 4 as well as alkaline pH of 9 inhibited the growth and nitrogen fixation.
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Subudhi et al. (1979) studied this question.
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