Reaction of Fish Insulins with Human Insulin Antiserums

VIRTUALLY all human subjects treated with insulin shortly develop circulating insulin-binding antibodies that are demonstrable by a variety of technics employing I131-labeled insulin.1 2 3 The formation of insulin-antibody complexes in human antiserums is a reversible reaction for which various kinetic and thermodynamic constants have been determined quantitatively.4 Insulin-binding capacities of antiserums from subjects that are not clinically resistant to insulin rarely exceed 10 to 20 units of insulin per liter of plasma whereas binding capacities of all but 1 of 54 antiserums from insulin-resistant subjects ranged from 57 to 4700 units per liter.5 Although individual antiserums showed similar insulin-binding capacities . . .