Chemical Constitution of N‐acylethanolaminephosphatides in Pea Seed

N‐Acylphosphatidylethanolamine (APE) and N‐acyllysophosphatidylethanolamine (ALPE) were isolated from pea seed and studied on the chemical constitution. The major component fatty acids of APE and ALPE were palmitic, oleic and linoleic acids. In both lipids the N‐acyl groups contained the same component acids, being more saturated than the O‐acyl groups. The 2‐C position of APE contained almost entirely unsaturated acids, and the 1‐C position mostly saturated acids. The 1‐acyl isomer of ALPE was richer in saturated fatty acids than the 2‐acyl isomer. The main molecular species of APE were 1‐palmitoyl‐2‐linoleoyl, 1‐palmitoyl‐2‐oleoyl, 1‐oleoyl‐2‐oleoyl, 1‐oleoyl‐2‐linoleoyl and 1‐linoleoyl‐2‐linoleoyl types. N‐Palmitoyl (or oleoyl and linoleoyl) glycerophosphorylethanolamine, which was the skeletal component of APE and ALPE, was confirmed as their TMS ether derivatives by GC‐MS.