Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI‐D) resistance by primer‐introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea

We have established a simple diagnosis method for rice blast fungus resistant to MBI‐D. This involves the preparation of PCR templates directly from the lesions in combination with primer‐introduced restriction enzyme analysis PCR (PIRA‐PCR).