An enzyme-linked immunosorbent assay has been developed for detecting antibodies combined with red cell surface antigens. In the test, the fluorescence released from 4-methylumbelliferylphosphate is measured after exposure of antibody-coated red cells to alkaline phosphatase coupled to anti-human globulin. The assay is sensitive, reproducible, quantitative and potentially adaptable to an automatic analyzer system.
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Osborne et al. (2009) studied this question.