What question did this study set out to answer?

Clarify the physiological stress tolerance and genetic diversity of soybean rhizobial isolates collected from diverse climatic and topographical soil regions in Venezuela.

January 1, 2019Microbes and EnvironmentsOpen Access

Burkholderia and Paraburkholderia are Predominant Soybean Rhizobial Genera in Venezuelan Soils in Different Climatic and Topographical Regions

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Authors

MRMaría Daniela Artigas RamírezBeijing Forestry University MEMingrelia EspañaUniversity of Hohenheim CAClaudia Aguirre

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Implication

Field study reveals Burkholderia and Paraburkholderia as predominant soybean rhizobia across diverse soils, suggesting novel beta-rhizobial symbionts for agricultural nitrogen fixation.

Key Points

Clarify the physiological stress tolerance and genetic diversity of soybean rhizobial isolates collected from diverse climatic and topographical soil regions in Venezuela.
Harvested 395 soybean root nodules from 11 soil types across different topographical regions to isolate 120 bacterial strains.
Screened all isolates for tolerance against NaCl and Al3+ stress, selecting 44 representative isolates for root nodulation verification and multilocus sequence typing (MLST).
Sequenced symbiosis-related genes (nodD–nodB intergenic spacer and nifH) and quantified nodule formation and nitrogen-fixation activity in soybean plants.
Multilocus sequence typing identified 24 of the 44 isolates as Burkholderia or Paraburkholderia, while 20 isolates belonged to the classical alpha-rhizobial genera Rhizobium and Bradyrhizobium.
All 44 isolates nodulated soybean, with five (Para)Burkholderia isolates forming higher nodule counts than any of the 20 alpha-rhizobia.
One Paraburkholderia isolate demonstrated the highest nitrogen-fixation capacity among all 44 evaluated isolates.

Cite This Study

Ramírez et al. (2019) studied this question.

synapsesocial.com/papers/6a978075883af1d6c8a66ccc https://doi.org/10.1264/jsme2.me18076

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Implication

Field study reveals Burkholderia and Paraburkholderia as predominant soybean rhizobia across diverse soils, suggesting novel beta-rhizobial symbionts for agricultural nitrogen fixation.

Burkholderia and Paraburkholderia are Predominant Soybean Rhizobial Genera in Venezuelan Soils in Different Climatic and Topographical Regions

Discussion

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Implication

Key Points

Cite This Study

Burkholderia and Paraburkholderia are Predominant Soybean Rhizobial Genera in Venezuelan Soils in Different Climatic and Topographical Regions

Discussion

Member takes

Implication

Key Points

Cite This Study

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