Free amino acids accumulated in non-thermodormant embryonic axes of New York lettuce seeds from the 16th to the 24th hour of imbibition, even when germination was prevented with 0.3 m mannitol. The accumulation of free amino acids in the embryonic axes was affected by temperature, red light and hormones (gibberellic acid, kinetin and abscisic acid), and was correlated with the growth potential of the axes. These data are evidence that the accumulation of free amino acids in the embryonic axes is not the result of axes-growth, but one of its causes.
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Go Takeba (1980) studied this question.