The aceto-iron hematoxylin method of Wittmann for algal chromosomes was improved by replacing the chloral hydrate and slide heating steps with 5 N HCl hydrolysis. Squashing of the unstained material under the coverslip followed by coverslip removal and air drying allowed rapid and more homogeneous staining. Additional procedures were developed to make the method easier and quicker. Good results were obtained for all algal species analyzed. Pit-connections of red algae were also clearly differentiated.
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Fujii et al. (1998) studied this question.
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