Microfluidic Multicolor Encoding of Microspheres with Nanoscopic Surface Complexity for Multiplex Immunoassays

Technicolor dreamcoating: A microfluidic device produces and manipulates double-emulsion droplets of equal size with unprecedented controllability (see picture). The droplets, which contain color core droplets, are photopolymerized to produce transparent microspheres. Silica colloidal particles anchored preferentially at the surfaces of the microspheres provide efficient binding sites for biomolecules for biological analyses.