Boronic acid functionalized g-C 3 N 4 nanosheets for ultrasensitive and selective sensing of glycoprotein in the physiological environment

nanosheets (B-g-CN) with a Wulff-type boronic acid feature, which selectively bind glycoprotein under physiological conditions. Meanwhile, the binding causes significant enhancement of the B-g-CN nanosheet fluorescence, providing the basis for glycoprotein sensing. With IgG as a model, a detection limit (LOD) of 2.2 nM (3σ/s, n = 11) was obtained within a linear range of 6.7-67 nM. The LOD was further improved to 52 pM subject to enrichment of the nanosheets, which well enables IgG assay in human urine samples. Moreover, it was successful in imaging endogenous and exogenous glycoproteins in living cells.