An antibody was developed that exhibited affinity for diosgenin as well as the spiroaminoketal alkaloids solasodine and tomatidine. Rabbits were immunized with a diosgenin conjugate prepared by succinylation of diosgenin, followed by purification with a silica solid-phase extraction cartridge and active ester conjugation to Limulus polyphemus hemocyanin (LPH). Rabbit serum was evaluated using an indirect competitive ELISA with diosgenin-BSA as the coating conjugate. A reduction in absorbance of 50% could be obtained using concentrations of ca. 0.8 μM diosgenin, 30 μM solasodine and 100 μM tomatine. The antibody exhibited no affinity for solanidine, a non-spiral glycoalkaloid.
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Driedger et al. (2001) studied this question.
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