An improved high-performance liquid chromatographic (HPLC) method for the determination of the potato glycoalkaloids, α-solanine and α-chaconine, is described. The glycoalkaloids were extracted with aqueous acetic acid, concentrated, and cleaned up by solid phase extraction using C 18 cartridge columns and separated using a reverse phase C 18 HPLC column. Recoveries averaged 93% ± 1.3 for α-solanine and 99% ± 3.1 for α-chaconine. Comparisons were made at each step with existing published methods. We conclude that trifunctional endcapped C 18 SPE cartridges were more reliable than traditional monofunctional non-endcapped cartridges and that low carbon load C 18 HPLC columns provided an improved separation of glycoalkaloids when compared to columns with a high carbon loading. An acidic extraction medium excluding organic solvents is recommended.
No takes yet. Share an insight, caveat, or question.
Edwards et al. (1996) studied this question.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: