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Methods for immobilizing glucose oxidase (GOx) on cellulose acetate (CA) membranes are compared. The optimal method involves covalent coupling of bovine serum albumin (BSA) to CA membrane and a subsequent reaction of the membrane with GOx, which has previously been activated with an ex¬ cess of p-benzoquinone. This coupling procedure is fairly reproducible and allows the preparation of thin membranes (5-20 irnl) showing high surface activities (1-3 U/cm2) which are stable over a period of 1-3 months. Electrochemical and radiolabeling experiments show that enzyme inactivation as negligible. A good correlation between surface activity of membranes and their GOx load
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Sternberg et al. (1988) studied this question.
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