Pt-doped FeP-C Hollow Nanorod and Hemoglobin Based Electrochemical Biosensor and Its Applications

Sensitive detections of nitrite (NO2-), hydrogen peroxide (H2O2), bromate (BrO3-) and trichloroacetic acid (TCA) have very important significance in different fields. Herein, Pt-doped FeP-C hollow nanorod was fabricated by a controllable etching and in-situ carbonating procedure. Then, a modified electrode (Nafion/Hb/Pt-FeP-C/CILE) was constructed by dropping Pt-FeP-C, hemoglobin (Hb) and Nafion membrane on carbon ionic liquid electrode (CILE). Benefitting from the synergistic effects of Pt NPs and FeP-C, Pt-FeP-C provides a favorable biocompatible microenvironment for Hb attachment with enhanced catalytic performance. Electroanalysis of NO2-, H2O2, BrO3- and TCA were realized in the detection range from 0.2 to 7.0 mmol·L-1, 0.3 to 7.0 mmol L-1, 0.76 to 7.0 mmol·L-1, 5.0 to 900.0 mmol L-1, and the detection limits were 0.07 mmol L-1, 0.1 mmol L-1, 0.25 mmol L-1 and 1.67 mmol L-1, respectively. The real samples were further analyzed with satisfactory results.