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A maximum fluorescence enhancement of 11 times was achieved by surface plasmon polaritons (SPPs) on a silver nanohole array region in reflection mode, compared to that on the nonarray area. A 30 nm dielectric SiO 2 film was sputtered on the silver film as a spacer to separate the fluorophore from silver for attenuation of the fluorescence quenching. An array period of 550 nm and a nanohole radius of 100 nm were optimized to match the most efficient fluorescence excitation and emission of a boron-dipyrromethene fluorophore (BODIPY 630/650) on the array region.
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Guo et al. (2009) studied this question.
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