Azinomycins A and B, new antitumor antibiotics. I Producing organism, fermentation, isolation, and characterization.

A strain of Streptomyces griseofuscus S42227 (FERM P-8443) was found to produce new antitumor antibiotics, called azinomycins A and B. The molecular formulas of azinomycins A and B were determined as C30H33N3O10 and C31H33N3O11, respectively.They were active against Gram-positive bacteria, Gram-negative bacteria and L5178Y cells in tissue culture.In the course of screening for new antitumor substances, azinomycins A and B (Fig. 1) were discovered in the culture broth of strain S42227.This paper reports the taxonomy of the producing organism, fermentation, isolation, physico-chemical properties, antimicrobial activity and in vitro cytotoxicity of azinomycins.The structures1) and antitumor activity2 of azinomycins will be published elsewhere.