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January 14, 2011Journal of Medical Genetics

Rapid detection of genetic variants in hypertrophic cardiomyopathy by custom DNA resequencing array in clinical practice

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Why the study?

Does a custom DNA resequencing array effectively detect pathogenic genetic variants in patients with hypertrophic cardiomyopathy?

Population

122 unrelated patients with hypertrophic cardiomyopathy (HCM)

Design

Cross-sectional

Key result

A custom DNA resequencing array identified known or novel potentially pathogenic heterozygous single-nucleotide variants in 31% of unrelated patients with hypertrophic cardiomyopathy.

Authors

SFSiv FokstuenPMPaola MelaciniSISabino Iliceto

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Overview

Should not yet change HCM genetic testing practice; leaves open array utility pending prospective validation.

Study Design

Type

Observational (n=122)

Structured PICO

Does a custom DNA resequencing array effectively detect pathogenic genetic variants in patients with hypertrophic cardiomyopathy?

P
Population
122 unrelated patients with hypertrophic cardiomyopathy evaluated using a custom DNA resequencing array.
E
Exposure
Custom DNA resequencing array interrogating all possible single-nucleotide variants on both strands of all exons, splice sites and 5'-untranslated region of 12 HCM genes
O
Outcome
Identification of known or novel potentially pathogenic heterozygous single-nucleotide variantssurrogate

A custom DNA resequencing array provides a rapid and efficient method for first-tier mutation screening in hypertrophic cardiomyopathy, detecting potentially pathogenic variants in 31% of patients.

Cite This Study

Fokstuen et al. (2011) conducted an observational in Hypertrophic cardiomyopathy (n=122). Custom DNA resequencing array was evaluated on Identification of known or novel potentially pathogenic heterozygous single-nucleotide variants. A custom DNA resequencing array identified known or novel potentially pathogenic heterozygous single-nucleotide variants in 31% of unrelated patients with hypertrophic cardiomyopathy.

synapsesocial.com/papers/6a97b90fd1113418b0fac9adhttps://doi.org/10.1136/jmg.2010.083345
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