A rapid and effective HPLC-DAD-MS method for quantitatively identifying the flavonoids of Apoacynum venetum L and Poacynum hendersonii has been developed. With this method, all the main components were well separated. In Apoacynum venetum L, the main components are chlorogenic acid, hyperoside, isoquercitrin, and 6″-O-malonylhyperoside, while those of Poacynum hendersonii are quercetin3-O-sophoroside, isoquercitrin and 6″-O-malonylhyperoside. The experimental results showed that the developed approach is accurate, reproducible, and applicable for quantifying the bioactive components of Apoacynum venetum L and Poacynum hendersonii.
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Shi et al. (2011) studied this question.
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