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The sequence of siomycin A was determined from the seventeen fragments of the modified antibiotic, although a complete structural formula is still not available. Siomycin contains eleven constituents, two of which are still unidentified. In the determination of the sequence, the reduced-, oxidized-, ammonia treated-siomycin A, partial acidic hydrolysis was used to provide water soluble fragments. Sanger's (DNFB) method was applied to the fragments to obtain sequence information. The structure surrounding the dehydroalanine moiety, the properties of the sulfhydryl group and the lactone ring were also investigated.
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Miyazaki et al. (1969) studied this question.
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