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In many fields of biology enzyme research falls into two general classes. First, there are studies which are primarily concerned with enzymes as such, their occurrence, distribution and properties. In these studies it is desirable to obtain as pure a preparation of the enzyme as possible and then to reconstruct the system and study its characteristics in vitro. The scientific worker interested in this phase of enzyme research does not usually study any one particular group of organisms or tissues but investigates many groups so as to determine the properties of the same enzyme as it occurs in different environments.
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Burris et al. (1939) studied this question.
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