The full assignments of the 13C NMR signals of the herqueinone-type phenalenone, herqueinone, were established by a combination of the single-frequency off-resonance decoupling technique and the 13C-enrichment technique using the 13C-labeled tracer. Furthermore, the full assignments of the 13C NMR signals of deoxyherqueinone-type, dihydroherqueinone-type, and xanthoherqueinone-type phenalenone were completed on the basis of the application of the chemical shift rule and the mutual comparison of their chemical shifts.
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Suga et al. (1983) studied this question.
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