Improved classification of alpha‐1‐antitrypsin in immobilized pH gradients containing sucrose

Two narrow immobilized pH gradients (pH 4.35–4.85 and pH 4.40–4.80) were formulated by linear interpolation from standard 1 pH unit ranges. Both gradients proved excellent for alpha‐1‐antitrypsin phenotype separation. Gel casting was simplified and the properties of the gels during the run improved by replacing glycerol with sucrose in the polymerization solutions.