Optimierung eines Enzymimmunoassay zum Nachweis von Antikörpern gegen aviäre Reoviren ‐ unter besonderer Berücksichtigung der Ergebnisberechnung und Testinterpretation

Selected steps in the establishment of an enzyme-immuno-assay (EIA) are shown with an EIA for detection of antibodies against avian reoviruses of chicken. The centre of this work is to show the various possibilities of calculation and interpretation of the test results. The definition of the cut-off-value is the basis for the interpretation of the assay and the consequences for the veterinarians in the field and the animal owners. Basic parameters (sensitivity, specificity, predication value, prevalence) of the test validation are discussed with the example of the examination of a new hen flock in the EIA, compared to serum neutralisation test.