A rapid ion-exchange chromatographic method for determination of total homocysteine in plasma is presented. The method has a coefficient of variation of approximately 5%. A comparison with a slower method previously used in our laboratory showed a high correlation (r=0.993). The risk of oxidation of homocysteine during the chromatography and the necessary level of dithio-treithol to prevent oxidation are also established.
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Andersson et al. (1989) studied this question.
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