Data consistency, yield, maintenance, and hysteresis in batch cultures of Candida lipolytica cultured on n‐hexadecane

Candida lipolytica was cultured batchwise using n‐hexadecane as the main carbon source. Biomass production, n‐hexadecane consumption, oxygen consumption, and carbon dioxide evolution were measured to follow the fermentation. The consistency of the measured data was examined using integrated and instantaneous available electron and carbon balances. Values of the “true” growth yield, ηmax, and maintenance coefficient, me were estimated using three different sets of data (biomass and n‐hexadecane, oxygen and biomass, and CO2 and biomass), and the results were compared with estimates obtained from literature data. Hysteresis patterns were observed in plots of specific rates of oxygen consumption and carbon dioxide evolution versus specific growth rate.