Flavonoids are ubiquitous in the plant kingdom and are very efficient antioxidants. High-performance liquid chromatography (HPLC) with electrochemical detection (ECD) is a sensitive, simple and selective method for determination of flavonoids. Figure 1 shows typical HPLC chromatograms and the legend gives details of the method of preparation of HPLC samples of flavonoids (catechin) from the plasma of rats given green-tea extracts. The major flavonoids (catechins) in green-tea extracts are the four epicatechins: (-)-epicatechin, EC; (-)-epicatechin gallate, ECG; (-)-epigallocatechin, EGC; and (-)-epigallocatechin gallate, EGCG. They were separated on a ODS C18 reversed-phase column by isocratic elution with 85:15 0.1% phosphoric acid-acetonitrile solution containing 0.1 mM Na2EDTA [1].
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Hideharu Shintani (2013) studied this question.