Key result
The detergent and enzyme extraction (DEE) method achieved complete decellularization and preserved the mechanical function and tissue structure of bovine pericardium better than TS or TSD methods.
Why the study?
Does the DEE method improve decellularization and preservation of mechanical function and tissue structure in bovine pericardium compared to TS and TSD methods?
Does the DEE method improve decellularization and preservation of mechanical function and tissue structure in bovine pericardium compared to TS and TSD methods?
The detergent and enzyme extraction (DEE) method is superior to TS and TSD methods for preparing decellularized bovine pericardium scaffolds for tissue-engineered heart valves by preserving mechanical function and tissue structure.
Answers are grounded in this paper and cite their sources.
Supports DEE scaffolds in preclinical valve engineering; leaves open human translation and in vivo testing.
Bovine pericardium has been extensively applied as the biomaterial for artificial heart valves and may potentially be used as a scaffold for tissue-engineered heart valves after decellularization. Although various methods of decellularization are currently available, it is unknown which method is the most ideal one for the decellularization for bovine pericardium. We compared three decellularization methods, namely, the detergent and enzyme extraction (DEE), the trypsin (TS), and the Triton X-100 and sodium-deoxycholate (TSD) method, to examine their efficacy on cell removal and their preservation of the mechanical function and the tissue matrix structure. Results indicated that decellularization was achieved by all the three methods as confirmed by hematoxylin-eosin staining, scanning electron microscopy, as well as quantitative DNA measurement. However, TS and TSD methods resulted in severe structural destruction of the bovine pericardium as shown by von Gieson staining and Gomori staining. Furthermore, both TS and TSD methods changed the mechanical property of the bovine pericardium, as evidenced by a lower elastic modulus, maximal-stress, maximal-disfiguration, maximal-load, and maximal-strain. In conclusion, the DEE method achieved both a complete decellularization and preservation of the mechanical function and tissue structure of the bovine pericardium. Thus, this method is superior to either the TS or the TSD method for preparing decellularized bovine pericardium scaffold for constructing tissue-engineered heart valves.
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Yang et al. (2009) studied Tissue-engineered heart valves. Detergent and enzyme extraction (DEE) method vs. Trypsin (TS) and Triton X-100 and sodium-deoxycholate (TSD) methods was evaluated on Efficacy on cell removal and preservation of mechanical function and tissue matrix structure. The detergent and enzyme extraction (DEE) method achieved complete decellularization and preserved the mechanical function and tissue structure of bovine pericardium better than TS or TSD methods.
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