By using the pump–probe technique, the femtosecond (fs) time-resolved absorption dynamics of oxyhaemoglobin (oxy Hb) in living erythrocytes was studied for the first time to our knowledge. The changing course of erythrocyte absorption at 400 nm was found to be composed of three exponential decay components with the time constants of 200±28 fs, 3.60±0.14 ps and 215±21 ps respectively, which are consistent with those of oxy Hb water solution measured in the same way, indicating that the fast process (less than 1 ns) of photo-induced response in erythrocytes was as the same as that in oxy Hb aqueous solution. These three decay components were assigned to the transitions from excited states of unligated haemoglobin (Hb* I , Hb* II ) and fast geminate recombination of O 2 and Hb, respectively.
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Cen et al. (2004) studied this question.
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