Key result
Hypotonic lysis and nuclease digestion preserved >80% of ATP and AMP hydrolytic activity on porcine aortic valves, whereas detergent decellularization completely removed (<5%) all ecto-enzyme activities.
Why the study?
Do different decellularization strategies affect extracellular nucleotide metabolism on porcine aortic valve xenografts?
Do different decellularization strategies affect extracellular nucleotide metabolism on porcine aortic valve xenografts?
Different decellularization techniques significantly alter extracellular nucleotide metabolism on porcine aortic valves, which may impact the durability and function of valve bioprostheses.
Detergent vs hypotonic decellularization differentially preserves valve ecto-enzyme activity; leaves open effects on clinical prosthesis thrombosis or calcification.
Extracellular nucleotide metabolism controls thrombosis and inflammation and may affect degeneration and calcification of aortic valve prostheses. We evaluated the effect of different decellularization strategies on enzyme activities involved in extracellular nucleotide metabolism. Porcine valves were tested intact or decellularized either by detergent treatment or hypotonic lysis and nuclease digestion. The rates of ATP hydrolysis, AMP hydrolysis, and adenosine deamination were estimated by incubation of aorta or valve leaflet sections with substrates followed by HPLC analysis. We demonstrated relatively high activities of ecto-enzymes on porcine valve as compared to the aortic wall. Hypotonic lysis/nuclease digestion preserved >80 % of ATP and AMP hydrolytic activity but reduced adenosine deamination to <10 %. Detergent decellularization completely removed (<5 %) all these activities. These results demonstrate high intensity of extracellular nucleotide metabolism on valve surface and indicate that various valve decellularization techniques differently affect ecto-enzyme activities that could be important in the development of improved valve prostheses.
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Kutryb-Zając et al. (2016) studied Aortic valve xenograft decellularization (n=12). Decellularization strategies (hypotonic lysis vs detergent treatment) vs. Intact native porcine valves was evaluated on Rates of ATP hydrolysis, AMP hydrolysis, and adenosine deamination. Hypotonic lysis and nuclease digestion preserved >80% of ATP and AMP hydrolytic activity on porcine aortic valves, whereas detergent decellularization completely removed (<5%) all ecto-enzyme activities.
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